PIM3,丝氨酸/苏氨酸蛋白激酶PIM3抗体

产品编号HZ-4852R
英文名称PIM3
中文名称丝氨酸/苏氨酸蛋白激酶PIM3抗体
别 名Kid 1; Kid-1; Kid1; Kinase induced by depolarization; ONCOGENE PIM 3; ONCOGENE PIM3; PIM 3; Pim 3 oncogene; PIM3; Pim3 oncogene; PIM3_HUMAN; Protein kinase Kid 1; Protein kinase Kid1; Serine/threonine kinase Pim 3; Serine/threonine kinase Pim3; Serine/threonine protein kinase Pim 3; Serine/threonine protein kinase Pim3; Serine/threonine-protein kinase Pim-3.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 信号转导 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human,
PIM3,丝氨酸/苏氨酸蛋白激酶PIM3抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量36kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PIM3
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PIM3,丝氨酸/苏氨酸蛋白激酶PIM3抗体PubMedPubMed
产品介绍background:
The Pim family serine/threonine protein kinases were first identified in studies examining genes targeted for proviral insertion in murine leukemia virus-induced T lymphomas. Increased levels of Pim kinases predispose cells to lymphomagenesis and enhance the activity of mitogenic proteins such as p100, c-Myb, and Cdc25A. In addition, Pim kinases are also involved in modulation of synaptic strength in neurons and anti-apoptotic signaling in hematopoietic progenitor cells. Pim-3, a member of the proto-oncogene Pim family that expresses serine/threonine kinase activity, shares significant homology with Pim-1 serine/threonine protein kinases. Pim-3 may function as a mediator of synaptic plasticity in the brain and is presumably involved in the anti-apoptosis process and cell cycle progression as well as the proliferation of human hepatoma cell lines. The Pim-3 protein is widely expressed, however no expression is observed in the colon, thymus, or small intestine.

Function:
Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.

Subunit:
Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM3, ubiquitination and proteasomal degradation. Interacts with SOCS6.

Subcellular Location:
Cytoplasm.

PIM3,丝氨酸/苏氨酸蛋白激酶PIM3抗体Tissue Specificity:
Detected in various tissues, including the heart, brain, lung, kidney, spleen, placenta, skeletal muscle, and peripheral blood leukocytes. Not found or barely expressed in the normal adult endoderm-derived organs such as colon, thymus, liver, or small intestine. However, expression is augmented in premalignant and malignant lesions of these organs.

Post-translational modifications:
Ubiquitinated, leading to proteasomal degradation.
Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.

Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain.

Gene ID:
415116

Database links:
Entrez Gene: 415116 Human
Entrez Gene: 223775 Mouse
Omim: 610580 Human
SwissProt: Q86V86 Human
SwissProt: P58750 Mouse
Unigene: 530381 Human
Unigene: Hs.530381 Human
Unigene: 400129 Mouse

PIM3,丝氨酸/苏氨酸蛋白激酶PIM3抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.